6/2/2023 0 Comments Imagequant tl 8.1 crack![]() Equal amounts of protein (35 μg) were separated by SDS-PAGE (10% SDS-polyacrylamide gel electrophoresis) and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad Laboratories, Hercules, CA). Protein concentration was determined using the NANOdrop Spectrophotometers. The dissolved proteins were collected after centrifugation at 10,000 g for 30 min, and the supernatant was then collected. Group IV Vitamin C + AF64A the animals were orally treated with vitamin C (250 mg/kg BW), a standard antioxidant that was previously reported to enhance memory and to attenuate neurodegeneration, once daily for a period of 14 days after the administration of AF64A. Group III Donepezil + AF64A the animals were orally treated with donepezil (Aricept) (1 mg/kg BW), a cholinesterase inhibitor that is widely used as a standard drug for dementia treatment, once daily for a period of 14 days after the administration of AF64A. Group II Vehicle + AF64A rats were orally treated with propylene glycol once daily for a period of 14 days after the administration of AF64A. Group I Vehicle + ACSF rats were orally given propylene glycol, which served as the vehicle to suspend the combined extract of CP1, once daily for 14 days after the administration of ACSF. ![]() The animals were allowed to recover from anesthesia and then placed in their cages. After being left at the injection site for 5 min, the needle was slowly withdrawn.
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